Teacher

School

Rec’d

Prep

Del

Ret

Assistance

Class #

Students/class

Groups

1

2

3

4

5

6

total

Number

Equipment/Supplies

Micropipettor KIT:
3or 4  sizes of pipettors
3 sizes micropipettor tips
(125 ml bottle methylene blue n/a this lab)
(4 small plastic cups n/a this lab

DME KIT:
2 – Electrophoresis Chambers
1 – Power Supply
2 -10ul fixed volume micropippettors
2 – 200μl tip boxes
2 – Flat Racks
(tube of salt – n/a this lab)
(set of dyes – n/a this lab)

Thermocycler (optional)

55° C water bath

Hot plate (boiling water bath)

PCR bead in pcr tube

100 bp ladder (1 aliquot per 6 groups)

12μ L of 0.05 ng/μl lambda DNA

6 μl of Primer Mix at 12 pm/μL

Sterile deionized water

Disposable gloves

Loading dye

1.2% agarose gels

1 X TBE buffer

0.025% methylene blue stain

Sharpie pen

Weighing boats for staining

Lab Sheets

Power Point Print-out/CD

Teacher Evaluation