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Amylase Adventure 
(Groups of 2)

Teacher

School

Recd

Prep

Del

Ret

Assistance

 

 

 

 

 

 

 

Class#

Students/class

Groups

1

 

 

2

 

 

3

 

 

4

 

 

5

 

 

6

 

 

total

 

 

  Number

Equipment/Supplies

 

Starch agar plates 1/group SOP BR

 

Starch nutrient plates 1  /group S0P BR

 

Coffee filters (1 per group, cut in half)

 

Iodine test solution (125 ml bottle)SOP BR

 

0.1M Tris Buffer (125 ml bottle)SOP BR

 

Distilled water (20 μl/group)

 

1 % starch solution (125 ml bottle)SOP BR

 

Ice

 

Digital balance (or available scales)

 

Spectrophotometer and cuvettes

 

Disposable pipettes or droppers 2 /group

 

Mortar & pestle

 

Sharpies or wax pencils

 

Test tubes (8) and rack per group

 

Sterile swabs (Q tips)  (1/group)

 

Weigh boats

 

25 ml graduated cylinder

 

100 ml fixed volume micropipette + tips

 

Large plastic cup

 

Potato, sweet potato, carrot, soaked beans, Baker’s yeast, Yogurt, apple, plant leaves or roots for extracting amylase  (teacher must provide)

 

Soil samples (students collect)

 

Lab protocol

 

Teacher evaluations