School

Recd

Prep

Del

Ret

Assistance

Class#

Students/class

Groups

1

2

3

4

5

6

total

  Number

Equipment/Supplies

Laemmli buffer (250ml /group) SOP, BR     Reuse

Sample preparation buffer (10ml/group) SOP, BR      Reuse

Real crab meat (1cm3 per group)

Imitation crab meat (1 cm3 per group)

Mary’s Crab Dish Extract (pre-measured) SOP, BR

Dan’s Crab Dish Extract (pre-measured) SOP, BR

Medium weigh boat for samples

Coomassie Blue staining solution SOP, BR      Reuse

Destaining Solution (vinegar base) SOP, BR

Large weigh boats for destaining

SDS – agarose gels SOP, BR

Mortar and Pestle kit

Transfer pipettes  (2/group)

Microcentrifuge

Microcentrifuge tubes (2/ group)
Different colors for extracts of real and imitation crab

 Hot plate with bubble rack

DME KIT:
2 – Electrophoresis Chambers
1 – Power Supply
2 -10μl fixed volume micropippettors
2 – 200μl tip boxes
2 – Flat Racks
(tube of salt – n/a this lab)
(set of dyes – n/a this lab)

Graduated cylinders   (25mL)

Deionized or Distilled  water   (1L)

Digital balance

Plastic spoons (1 per group)

Metric ruler

White light box or overhead projector

Lab sheets

Teacher evaluation