Standard Operating Procedure

Subject: Preparing Laemmli Buffer

DATE: 21 November

SOP #SFF1

Prepared By: Christy Ackley

Approved By: Tarea Sanders

Page 1 of 1

Suggested vendors, catalog numbers, current prices:

SDS SDS

Strength/Concentration_____________ Date______________ Daigger FX449428GCJ 50mL $2.27

Company: ___________ Lot#_________ Cat#________

Tris Tris

Strength/Concentration_____________ Date______________ Fisher Scientific BP152 500g $57.10

Company: ___________ Lot#_________ Cat#________

Boric Acid Boric Acid

Strength/Concentration_____________ Date_______________ Daigger FX448684GCC 500g $8.74

Company: ___________ Lot#_________ Cat#_________

LAEMMLI’S BUFFER:

· Prepare 1L of Laemmli buffer; store at room temperature indefinitely

Instructions:

1. Combine 10mL 10% SDS, 11.0g Tris base, and 6.0g Boric acid in a 2L flask

2. Bring to volume with 1L deionized water

3. Swirl until completely dissolved