Standard Operating Procedure

Subject: Preparing Dan’s Crab Dish Extract

DATE: 21 November 2006

SOP # SFF3

Prepared By: Christy Ackley

Approved By: Tarea Sanders

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DAN’S CRAB DISH EXTRACT:

· Make 0.5ml extract of Dan’s Crab Dish; store in the freezer for up to one month

Instructions:

1. Combine 2.5g imitation crabmeat and 5mL cold, deionized water

2. Grind with a mortar and pestle

3. Add 7.5mL of sample preparation buffer to the mortar; stir to combine

4. Transfer the sample into two 15mL centrifuge tubes; centrifuge for five

minutes at 3000rpm

5. Remove the supernatant from each of the tubes and combine into one test tube.

6. Dispense 0.5mL aliquots into 1.5mL microcentrifuge tubes and puncture the

top of each microcentrifuge tube

7. Add the microcentrifuge tubes to a float and place in a 95 degree Celsius water bath for three minutes, this will denature the proteins

8. Remove the microcentrifuge tubes from the water bath and let cool

9. Seal the tubes with plastic wrap