Batch Record

Subject: Family Secrets Samples

Date: 06 October 2008

Approved By: Megan Waugh

Sample Dyes

Erioglaucine

Company:_________________Lot #_____________Catalog #_____________

Xylene Cyanol

Company:_________________Lot #_____________ Catalog #_____________

Bromophenol Blue

Company:_________________Lot #_____________ Catalog #_____________

Glycerol

Company:_________________ Lot #_____________ Catalog #_____________

Initials Date

50% Glycerol:

1. Add 10mL of glycerol to 10mL of diH₂0 and mix well. _____ _____

Positive Sample

1. Measure 0.02g of Erioglaucine, 0.02g Xylene Cyanol,

and 0.02g Bromophenol Blue to 15mL of diH₂0 and

mix well. _____ _____

2. Add 5mL of 50% glycerol to the dye mixture and

mix well. _____ _____

Negative Sample

1. Measure 0.02g Erioglaucine and 0.02g Bromophenol Blue to

15 mL of diH₂O and mix well. _____ _____

2. Add 5 mL of 50% glycerol to the dye mixture and

mix well. _____ _____

Aliquot positive sample mixture into Positive Control, Jeremy, and Dad.

Aliquot negative sample mixture into Negative Control and Jenny.