Standard Operating Procedure

Subject: Preparing DNA Extraction Kit Contents

DATE: 20 November 2006

SOP: DNAX1

Prepared By: Christy Ackley

Approved By: Tarea Sanders_

Page 1 of 1

Suggested Vendors, Catalog number, current price:

Ethanol

Daigger FX449718RAN 1L $8.75

Papain

Daigger FX449210GAB 100g $39.76

WHEAT GERM

Instructions:

1) Purchase raw wheat germ at a health food store

2) Aliquot 1.5ml of raw wheat germ into a green microcentrifuge tube; store at 4°C for 1 month

SALT

Instructions:

1) Purchase non-iodized or iodized table salt at retail store

2) Aliquot 1.5ml of table salt into a blue microcentrifuge tube; store at room temperature indefinitely

DISHWASHING LIQUID

· Prepare a 50:50 solution; store at room temperature

Instructions:

1) Purchase preferably clear dishwashing liquid at a retail store

2) Prepare 50:50 solution by adding 100ml dishwashing liquid to 100ml distilled water

3) Aliquot 1.5ml of the mix into a pink/red microcentrifuge tube

ETHANOL

· Prepare a 70% solution or purchase 70% ethanol; store at 4°C indefinitely

Instructions:

1) To prepare 70%, add 700ml of ethanol to 300ml of distilled water and mix well

2) Dispense about 7ml into 15ml conical centrifuge tubes

PAPAIN

Instructions:

1) Weigh out 6g of papain granules per 100ml of distilled water to make a concentrated stock solution; store at 4°C for up to 60 days

2) Set the heat on low and stir to make an extract solution

3) Filter through a coffee filter to remove crude

4) Filter sterilize, and then dilute stock 1:10 with sterile distilled water to make a “ready-to-use” solution; store at 4°C for up to 60 days

TO PREPARE DNAX KIT:

• In a snack size Ziploc bag enclose:

1) Two 2oz plastic cups

2) One 1ml transfer pipet

3) One tube of wheat germ, salt, soap, and papain

Store the ethanol separately, for it may leak out

TO PREPARE DNAX EXTENSION:

• Send 1 more tube of each of the items listed above