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Demystifying Electrophoresis
(Groups of 4)

Teacher

School

Recd

Prep

Del

Ret

Assistance

 

 

 

 

 

 

 

Class#

Students/class

Groups

1

 

 

2

 

 

3

 

 

4

 

 

5

 

 

6

 

 

total

 

 

            Number

Equipment/Supplies

      

DME KIT:
2 – Electrophoresis Chambers
1 – Power Supply
2 – 10µl pre-set Micropipettes
2 – 200μl tip boxes
1 – Tube of salt
2 – Flat Racks
2 –Set of dyes: SOP, BR stock dyes, BR sample dyes
Methyl Orange
Bromophenol Blue

Xylene Cyanole
Pyronin
Safranin O
Unknown

 Gallon Deionized Water

 Liter Bromothymol Blue-0.25 L/group SOP BR

  Liter TBE Buffer-0.5 L/group REUSE SOP BR

1% TBE agarose gels - 1/group SOPmaking BRmaking, SOPpouring gels, BR pouring gels.

Lab sheets

PowerPoint print out

Teacher evaluation