Standard Operating Procedure
Subject: Preparing Bromophenol Blue Solution
DATE: 27 November 2006
SOP # Amax1
Prepared By: Christy Ackley
Approved By: Tarea Sanders
Page 1 of 1
Strength/Concentration________________ Date_____________ Suggested Vendors,
Catalog number, current price:
Company: ________________
Daigger FX448697GCL 5g
$13.85
1 mM BROMOPHENOL BLUE STOCK SOLUTION:
·
Makes 100ml; store at room
temperature indefinitely
Instructions:
1.
Weigh out 0.0692g of bromophenol blue (F.W. 691.9) to 100ml of distilled water
1.
Stir till dissolved
18.6 μM BROMOPHENOL BLUE SOLUTION:
·
Makes 1000ml; store at room
temperature indefinitely.
Instructions:
1.
Add 18.6ml of 1 mM stock to 981.4ml of distilled water
2.
Stir to dissolve