Tissue Culture Methods

COURSE: Tissue Culture Methods
COURSE NUMBER: BIOT 2921
INSTRUCTOR: F. Janiak-Spens
PHONE: (405) 682-1611, ext. 7414

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COURSE DESCRIPTION
Prerequisites: (W) (M), BIO 2125.
1 CREDIT. The student will earn to successfully maintain tissue culture cells in a healthy uncontaminated state for an extended period of time. The course will include making tissue culture media, monitoring cell growth, freezing cells, and bringing up frozen cells.

COURSE PACING

BIOT 2922 is a combination of a group-paced and individually-paced course. Your instructor will establish objectives to be met within the time parameters of the semester. These dates must be observed in order to maintain good standing in the class.

COURSE GRADING CRITERIA

A: 90-100% average
B: 80-89% average
C: 70-79% average
D: 65-69% average
F: Below 65% average
Grades will be based on your level of mastery of the course objectives.

SOURCE OF CRITERIA
The objectives and competency levels for this course are based upon the professional judgment of Biotechnology faculty and upon comparable courses from other institutions of higher education.

ATTENDANCE POLICY:
You are expected to attend and actively participate in all scheduled classes for this course.

ACCOMMODATIONS FOR STUDENTS WITH SPECIAL NEEDS

Oklahoma City Community College complies with Section 504 of the Rehabilitation Act of 1973 and the American with Disabilities Act of 1990. Students with disabilities who need accommodations should make their request in the following ways:

· Talk with your instructor after class or during office hours about your disability or special needs related to work in class, AND
· Complete the Services to Students with Disabilities Intake/Referral Form provided in the Office of Services to Students With Disabilities (unless this has been completed).
· For additional information, call 682-7250 V/TTY

Tissue Culture

1. The student learns to make tissue culture media: to filter-sterilize components, combine, adjust pH and then to test it for sterility.

2. The student learns to monitor cell growth by observations of tissue culture medium changes and by observations of cells with inverted phase microscope.

3. The student learns to use the hemocytometer to count viable cells, and will perform counts at least three times.

4. The student learns to calculate cell concentrations from cell counts, and to calculate amounts to transfer to maintain optimum cell concentrations for running stock and for freezing.

5. The student learns and uses aseptic transfer skills in laminar flow hood .

6. The student learns and successfully performs procedures for freezing down cells and bringing up cells from the frozen state.

7. The student learns to maintain tissue culture cells in healthy uncontaminated state for a period of several weeks, using the skills listed above. The student will maintain his/her cell line for at least a week in PSF-free media without contamination

8. The student can distinguish between distilled and deionized water. The student can list all the purification steps found in our water purification system and describes briefly what each step accomplishes. The student can explain what is meant by type I water, and why it cannot be stored for any length of time. The student can describe how the quality of type I water is monitored.

9. The student will perform the above tissue culture procedures on two different cell lines: suspension mouse myeloma cells and adherent CHO cells.

10. The student will maintain acceptable documentation of all manipulations, observations, and procedures in a scientific notebook.

Oklahoma City Community College Biotechnology Program
Dr. Fabiola Janiak-Spens, Program Director
7777 S. May Avenue
Oklahoma City, OK 73159-4444
(405) 682-1611, ext 7414

Comments: Fspens@occc.edu

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