COURSE: MEDIA
AND SOLUTION PREPARATION
COURSE NUMBER: BIOT
1022
PHONE: (405)
682-1611, ext.
OFFICE HOURS:
COURSE
DESCRIPTION
Prerequisite:
(W) (M), College Biology, CHEM 1115
Corequisite: BIO 2125
2 CREDITS. The student will prepare media and solutions,
use calculations required for solution preparation, and use equipment
for solution preparation such as the analytical balance, pH meter, and
autoclave.
COURSE
COMPETENCIES
The student will learn to prepare media and solutions
in a context that relates to the job functions of a bioscience technician:
performing tests, making products, obtaining and processing materials,
controlling inventory, using and maintaining equipment and facilities,
performing and documenting safe practices, complying with regulations,
maintaining quality assurance, evaluating and documenting results, and
communicating information.
COURSE
PACING
BIOT 1022 is a group-paced course. Your instructor will establish testing and assignment
dates. These dates must be observed
in order to maintain good standing in the class.
COURSE
GRADING CRITERIA
A: 90-100%
average
B: 80-89%
average
C: 70-79%
average
D: 65-69%
average
F: Below
65% average
Grades will be based on your performances on a
combination of classroom tests, presentations, and assignments.
SOURCE
OF CRITERIA
The objectives and competency levels for this course
are based upon the professional judgment of Biotechnology faculty and
upon comparable courses from other institutions of higher education,
as well as the national skill standards for a bioscience technician.
LEARNING
RESOURCES
Seidman and Moore: Basic Laboratory Methods for
Biotechnology. Class
Handouts.
ATTENDANCE
POLICY:
You are expected to attend and actively participate
in all five scheduled classes for this course. Text reading will not be a satisfactory substitute
for participating in laboratory work.
ACCOMMODATIONS
FOR STUDENTS WITH SPECIAL NEEDS
Oklahoma City
Community College
complies with Section 504 of the Rehabilitation Act of 1973 and the
American with Disabilities Act of 1990.
Students with disabilities who need accommodations should make
their request in the following ways:
Talk with your instructor after class or during
office hours about your disability or special needs related to work
in class, AND
Complete the Services to Students with Disabilities
Intake/Referral Form provided in the Office of Services to Students
With Disabilities (unless this has been completed).
For additional information, call 682-7250 V/TTY.
DETAILED
COURSE OBJECTIVES
Job
Function “Performing Tests”
1.
Obtain and read SOP, protocol:
The student will read procedures in preparation for laboratories.
2. Assess acceptability of specimens: The
student will perform aseptic transfers of bacteria. The student will
streak for isolation. The student
will inoculate cultures, assess for purity, and maintain a pure culture
over the period of the course and freeze culture.
Student will assess a
frozen culture for viability. The
student will perform serial dilutions for plate counts, with proper
use of pipettes.
Job
Function “Manufacturing Products”
1.
Follow SOP, protocol:
the student will follow the written procedures in the laboratory.
2.
Manipulate data:
The student will plot and analyze data.
3. Perform
calculations: Student will be able to calculate grams of reagent
needed to make solution of specific molarity and volume.
Student will be able to calculate number of grams
needed for a given Normality.
Student will be able to calculate the number of
grams needed to make a %(weight/volume) solution.
Student will do calculations to prepare solutions
from stock solutions (dilutions).
The student will be able to make a buffer of given
pH.
The student will be able to calculate original
concentrations of organisms from results of serial dilution plate counts.
4. Obtain and Store product samples: Student
will correctly use balances, pH meters, and volumetric apparatus to
make, dispense, and properly store solutions.
The student will correctly use balances, pH meters,
volumetric apparatus, autoclave/filter sterilization, and aseptic technique
to make, dispense, and store media.
Student will monitor physical properties of a solution,
pH of solutions, the sterility of media, what counts for a pure culture.
Job
Function “Obtaining and Processing Materials”
1.
Obtain and label materials:
The student will properly label all plates, bottles, tubes during lab
procedures.
2.
Assess acceptability of reagents:
Student will read labels to check expiration dates
and lot numbers on bottles.
Student will maintain reagent integrity.
Job
Function “Controlling Inventory”
Control inventory:
Student will report when supplies or materials
are at low levels.
Student will demonstrate cost-awareness by effective
use of laboratory resources.
Job
Function “Maintaining Equipment and Facilities”
Maintain clean, orderly work environment:
Student will clean working areas according to SOP.
Student will return unused supplies to proper location.
Students will clean and properly store glassware
and equipment.
Students will use appropriate techniques when using
a pH meter, balance, microscope, autoclave, micropipettors, glassware.
Job
Function “Performing and Documenting Safe Practices”
Perform and document safe practices:
Student will disinfect work areas before and after
using.
Student will follow OSHA universal precautions
for biological pathogens.
Student will use protective equipment when necessary.
Student will properly handle, contain, and dispose
of bio-hazardous materials.
Student will know the procedures for handling,
containing, and disposing of radioactive
materials.
Student will know the location of eyewash station,
fire extinguisher, and First Aid supplies.
Student will know and perform safe operation of
the autoclave.
Student will maintain and follow chemical hygiene
plan.
Student will maintain, understand, follow MSDS.
Student will recognize and avoid common lab hazards.
Student will recognize safety symbol signs.
Job
Function “Complying with Regulations”
Regulatory compliance:
Follow NIH and OSHA regulations.
Know and Use GLPs.
Job
Function “Maintaining Quality Assurance”
Check integrity of the product, procedure, specimen,
materials:
Students establish, maintain, and test reference
cultures.
Students test media, buffers, solutions for sterility,
pH, physical properties.
Students will maintain QA logs for cleaning, disinfection,
incubation, storage, waste disposal, and tracking of laboratory procedures.
Student will compare results to standards, controls.
Job
Function “Evaluating and Documenting Results”
Evaluation and Documentation of results:
Student will maintain a laboratory notebook.
The student will record all data in the notebook
in real time as it is generated.
Student will collect and graph data and evaluate
validity of results.
Student will identify abnormal results.
Tracking sheets and logs will be witnessed by a
fellow student, and signed off by instructor.
Job
Function “Communicating Information”
Written communication:
The student will keep a lab notebook.
The student will write or update protocols.
Oral communication.
The student will make oral presentation.
The student will effectively coordinate tasks with lab partner(s).
The student will notify appropriate persons about problems and observations.
The student will interact with colleagues to solve problems.
