Content	Activities	Resources
Introduction to Biotech Defining biotechnology, old and new History Overview Agriculture: crops, animals Health – vaccines, drugs. Environmental Industrial Bioethics Careers in Biotech - introduction	Class creates timeline. Making Cheese or Making Yogurt Students look over jobs in biotech. Career exploration	PPT Intro - what is biotechnology? Biotech timeline: DNA interactive site– go to “timeline” at top of menu; see also BIO timeline Video – World of biotech Search engine on Access Excellence web site will provide several versions. Click here for more resources Videos – OCCC has a few careers videos for loan. Go here to view short videos of research technicians talking about their jobs - click on Ron and Brian. Students do Careers in Biotech exercise.
DNA Basics Structure Properties, Location Function	Review DNA structure. Construct model of DNA. Extract DNA. Review DNA replication, transcription and translation.	Discovery of structure – go to DNA interactive and click on “code” Start with basics. Then go to DNA from the beginning, units 15-20. Create a virtual DNA model, a Lego model, a paper model, or a candy model. Use BBD protocol with extensions. Virtual replication and Virtual transcription and translation. Model protein synthesis – click on DNA workshop.
Lab Skills for Biotech Standards for laboratory skills Laboratory Safety Laboratory Notebooks Intro to Lab basics: Mass measurements. Volume measurements. Electrophoresis Techniques Solutions Compounds Molarity Dilutions Metric Review Scientific notation	Biotech skill standards Lab Safety Activities How to keep Lab NB Measurements: Using a Balance. Using micropipettors. Making Gels Intro to Electrophoresis with dyes. Application - newborn screening & sickle cell. Practice with dilutions & using spectrophotometer.	Click here for industry skill standards – various sources. Sample lab safety handout. Group activity on lab safety. Sample handout for lab notebook and grading notebooks. Exercise on using balance. Micropipetting – ppt and learning to use . For more practice, go to Micropip. exercises and class micropipetting challenge activity: It's in My Genes. PPT on Intro to electrophoresis. PPT on making a gel. Demystifying Electrophoresis Animation on electrophoresis Huntington's Case Study Sickle Cell Module, part III. Spec 20 assay lab with serial dilutions – click on Amax module. Graphing with Excel. Chemistry tutorials. Click here for more teaching and learning resources.
Cells Structure and Function Procaryote vs Eucaryote Cells and Cell Parts. Microbes	DNA extraction extension Inside the Cell. Working safely with bacteria.	DNA extraction exercise Cells Alive web site. Incredible animation of inside a cell. Amazing cells: video, interactive, cell parts, membranes, signalling Working with bacteria.
Bioinformatics Introduction to Bioinformatics	Web based activities. Using BLAST to identify bacteria. Inquiry exercise using HIV sequences. Inquiry web exercises in relatedness of organisms & evolution	Using zoology or biomedical examples. Virtual lab at HHMI for bacterial identification. HIV/AIDS bioinformatics. ENSI site – scroll to Investigating Evolutionary Relationships using databases. More internet resources.
Environmental Biotech Biodiversity Bioremediation	Microbes and oil spills	OERB hungry microbes expt. Biodegradable Packing Peanuts activity. Click here for more resources.
DNA – Genetic Engineering Restriction Enzymes. Plasmids. Transformation	Desktop activities. Make plasmid/RE model with Velcro. DNA Fingerprinting. Tfo experiment. Plasmid recovery. Plasmid identification.	DNA Scissors. Recombinant DNA Plasmids. DNA Goes to the Races. DNA Patterns. Assessment on understanding DNA patterns. Animation on restriction enz Go to DNA interactive –scroll down to Techniques, cutting & pasting for restr.enz., trf & sorting for transformation. Transformation with pGREEN Plasmid miniprep. Restriction analysis of plasmid
Proteins Amino Acids Enzymes Separation and Identification Purification Classic Video from 1970s - Paul Berg's Protein synthesis (fun!)	GFP as model protein Enzyme Activity Protein electrophoresis Protein Purification Protein synthesis	Scroll down to GFP – Green Light for Bio. Amylase Adventure lab. Seafood Forensics – scroll down to SFF module. Introduction to Column Chromatography Recovery of GFP by chromat. Paul Berg's protein synthesis in action
Forensics and biotech	DNA Typing. Who-Done-It ? Barnyard Crime scene Mitochondrial DNA Polymerase Chain Rxn	Go to VCU and scroll to “Justice DNA.” Desktop – DNA typing. CLUE game for class. ImmunoAssay –module page, click on Immunoassay. DNA Patterns-advanced Determination of Molecular Size by Gel Migration Measurements. Seafood Forensics also works in this unit. Mystery of the Romanovs exercise Animation of PCR Virtual PCR lab. Desktop PCR activity. Basic PCR -water bath & thermal cycler versions . More forensic resources.
Agricultural Biotechnology	Transgenic plants Inquiry – GM foods What is Bt corn. GM Foods – Lateral flow strip test. Testing for Bt corn	Methodology. GM foods web-based exercise. Video on Plant biotech. Animation: scroll down to lateral flow strip test. Perform PCR to detect GM corn . More internet resources on Ag biot.
Introduction to Human Genome Project	What is HGP? DNA sequencing. Students identify own genotype. Genetics and genomics resources. Microarrays and Epigenetics.	HGP web exercise and video. View animations at DNALC. Lots of resources on DNA interactive under “Genome.” ALU PCR laboratory. Alu consent form. PTC PCR laboratory. PTC consent form. http://www.genome.gov/Education/ Click here for more HGP resources. Inexpensive lab activity simulating the use of microarray to assess gene expression in breast cancer patients to determine best treatment. Click on teacher's guide.
Bioethics What is ELSI? What is Cloning? What about Stem Cells?	Societal issues. Eugenics Gene therapy	Visit this site to discover some issues. Video on bioethics. The dark side of genetics –last video on playlist. See also DNALC A vision for medicine in the future. Go through these exercises on cloning Huntington's disease Case Study. Try out these activities to understand the stem cell issues. Click here for more on bioethics.
Business of Biotechnology Biotechnology company Making a biotech product. Writing a Resume Interviews Careers	Makeup of a biotech company. Product development activity. Student writes resume for lab position. Mock job interviews.	Amylase Videos – OCCC has a few careers videos for loan. Students do Careers in Biotech exercise. Click here for more internet resources on careers. A range of biotech careers can be found at the Washington state life science site.