SOP (Standard Opertating Procedure

Spectrafuge Microcentrifuge

1. Plug in power cord from micro centrifuge into the wall socket.

2. Press on lid switch (located at bottom right) to open the lid. You should hear a click when it is opened correctly.

3. Remove plastic cap off the rotor by gently pulling up on the stainless steel knob.

4. Before placing your tube in the sample container double check the tube! Make sure it’s snapped down tightly.

5. Tubes must be in balanced configuration before running. This means you

must place a tube of similar size and sample volume (can use water) opposite each tube.

6. Replace the plastic cap on the rotor and close the outer lid.

7. Set the speed and time (these will vary with each procedure):

• The speed control knob (smaller & located bottom left) measures rpms. Each increment is 1000 rpm, so to set it for 3000 rpm, you’d set it on 3.

• The time control knob (larger & located bottom right) measures minutes. Set it according to the lab directions. *Note: for quick spins (no set speed/time) just use the quick spin switch (located on bottom left). However you must continually hold the switch down for the desired time you want. Quick spins are used to pool all liquids in the tube to the bottom for good mixing.

8. When the run is over, you can open lid, remove plastic cap and remove your sample tubes. Then replace the plastic cap over the rotor and close the outer lid when finished.