2. Place 100g canned beans and 200 mL of water into a blender and blend until smooth, adding water if necessary.
3. Filter the mixture through a coffee filter, discard the filter paper.
4. Repeat steps 2 and 3 for the soaked dried beans.
5. Prepare the Beanoâ solution by mixing 10 drops of Beanoâ with 24 mL water in a beaker.
6. Obtain 4 test tubes and label as follows:

Tube 1 (Beanoâ + Canned Beans)
Tube 2 (Beanoâ + Canned Beans Control)
Tube 3 (Beanoâ + Soaked Dried Beans)
Tube 4 (Beanoâ + Soaked Dried Beans Control)

7. Add 4 mL of Canned Bean Solution to Tubes 1 and 2, and 4 mL of Soaked Bean Solution to Tubes 3 and 4.
8. Take a baseline glucose reading for all tubes.  Add a drop of liquid from each tube to a glucose detection strip, wait the required time (as per the manufacturer) {usually 10-30 seconds}, and compare to the color guide for the glucose detection kit.  This is your reading for Time “0.”  (some brands will give values for light medium or dark while others give actual quantities in mg/dL)

9. Record your readings in mg/dL or by color (light, medium, dark) and on your data page.
10. Use a clean pipette to add 2 mL of Beanoâ Solution to Tube 1, pipette the mixture up and down to make sure it is mixed will.
11. Using a clean pipette for each tube, repeat step 9 for Tube 3.
12. Add 2 mL of water to Tubes 2 and 4.
13. Let the tubes sit for five minutes.
14. At the end of five minutes, take another glucose reading for each tube as in step 7.
15. Continue this cycle for a 15 minute period.

Clinistix

*Adapted from the Shoestring Biotechnology project by DeAnn Campbell and Megan Waugh, Oklahoma City Community College, BBDiscovery Project