Instructions: Pouring Gels

1. Check to see that liquid agar has cooled to about 50 degrees C (comfortable handling temperature.)

2. If using casting trays place rubber stoppers/dams on each end.

3. Next place comb on the casting tray by sliding it into the proper slots with big teeth facing down. Follow the lab directions to find out where to place the comb (i.e.: at either one end or in center of tray.) If using a Petri dish the comb will only fit on the center of the dish.

4. Measure 25 mL of agar and pour into casting tray or Petri dish. Agar should be about 1/3 the way up the “teeth” of the comb.

5. Check for bubbles. Bubbles are troubles! Simply pop them with the end of a paper clip.

6. Let it sit undisturbed for about 15-20 minutes while it solidifies.

7. When gel is cloudy throughout, it is done.

8. Remove comb gently, can place a small amount of buffer on the gel, from the gel. Next remove the 2 rubber stoppers off each end of the tray.

9. Gels prepared in casting trays can (1) be left on tray and placed directly inside the gel box and run, or (2) can be GENTLY removed from tray and placed directly on the platform of the gel box.

10. There is a correct way to place gels in boxes. Follow instructions:

“Electrophoresis System/ Gel Box”

(*Note: GELS CAN BE STORED IN THE SAME BUFFER, REFRIGERATED, AND USED AT A LATER DATE.)